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Selected Publications




Mechanism of 2′-fucosyllactose degradation by human-associated Akkermansia

L Padilla, AD Fricker, E Luna, B Choudhury, ER Hughes, ME Panzetta, ...

Journal of Bacteriology, e00334-23


The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protecting Chlamydia effectors from degradation

RJ Bastidas, M Kędzior, RK Davidson, SC Walsh, L Dolat, BS Sixt, ...

eLife 12


The emerging complexity of Chlamydia trachomatis interactions with host cells as revealed by molecular genetic approaches

RJ Bastidas, RH Valdivia

Current Opinion in Microbiology 74, 102330


A genetic system for Akkermansia muciniphila reveals a role for mucin foraging in gut colonization and host sterol biosynthesis gene expression

LE Davey, PN Malkus, M Villa, L Dolat, ZC Holmes, J Letourneau, ...

Nature Microbiology, 1-18


The Chlamydia effector CpoS modulates the inclusion microenvironment and restricts the interferon response by acting on Rab35

K Meier, LH Jachmann, G Türköz, MR Babu Sait, L Pérez, O Kepp, ...

Mbio 14 (4), e03190-22


Chlamydia repurposes the actin-binding protein EPS8 to disassemble epithelial tight junctions and promote infection

L Dolat, VK Carpenter, YS Chen, M Suzuki, EP Smith, O Kuddar, ...

Cell Host & Microbe 30 (12), 1685-1700. E10


The bacterial effector GarD shields Chlamydia trachomatis inclusions from RNF213-mediated ubiquitylation and destruction

SC Walsh, JR Reitano, MS Dickinson, M Kutsch, D Hernandez, ...

Cell Host & Microbe 30 (12), 1671-1684. e9


Genotypic and phenotypic diversity among human isolates of Akkermansia muciniphila

B Becken, L Davey, DR Middleton, KD Mueller, A Sharma, ZC Holmes, ...

Mbio 12 (3), 10.1128/mbio. 00478-21


Application of a Chlamydia trachomatis expression system to identify chlamydia pneumoniae proteins translocated into host cells

I Yanatori, K Miura, YS Chen, RH Valdivia, F Kishi

Journal of Bacteriology 203 (11), 10.1128/jb. 00511-20


An endometrial organoid model of interactions between Chlamydia and epithelial and immune cells

L Dolat, RH Valdivia

Journal of cell science 134 (5), jcs252403


A renewed tool kit to explore Chlamydia pathogenesis: from molecular genetics to new infection models
L Dolat, RH Valdivia
F1000Research 8

Ptr/CTL0175 is required for the efficient recovery of Chlamydia trachomatis from stress induced by gamma-interferon
ME Panzetta, AL Luján, RJ Bastidas, MT Damiani, RH Valdivia, HA Saka
Frontiers in Microbiology 10, 756

Chlamydia trachomatis fails to protect its growth niche against pro-apoptotic insults
BS Sixt, C Núñez-Otero, O Kepp, RH Valdivia, G Kroemer
Cell Death & Differentiation 26 (8), 1485

The Expanding Molecular Genetics Tool Kit in Chlamydia
RH Valdivia, RJ Bastidas
Journal of Bacteriology 200 (24), e00590-18


A Chlamydia effector combining deubiquitination and acetylation activities induces Golgi fragmentation
JN Pruneda, RJ Bastidas, E Bertsoulaki, KN Swatek, B Santhanam, …
Nature Microbiology 3 (12), 1377

An atlas of genetic variation linking pathogen-induced cellular traits to human disease
L Wang, KJ Pittman, JR Barker, RE Salinas, IB Stanaway, GD Williams, …
Cell Host & Microbe 24 (2), 308-323. e6

Site-specific glycosylation regulates the form and function of the intermediate filament cytoskeleton
HJ Tarbet, L Dolat, TJ Smith, BM Condon, ET O’Brien III, RH Valdivia, …
Elife 7, e31807

Chlamydia trachomatis fails to protect its growth niche against pro-apoptotic insults
BS Sixt, C Núñez-Otero, O Kepp, RH Valdivia, G Kroemer
Cell Death & Differentiation 26 (8), 1485


The effector TepP mediates recruitment and activation of phosphoinositide 3-kinase on early Chlamydia trachomatis vacuoles
V Carpenter, YS Chen, L Dolat, RH Valdivia
MSphere 2 (4), e00207-17

Chlamydia trachomatis’ struggle to keep its host alive
BS Sixt, RH Valdivia, G Kroemer
Microbial Cell 4 (3), 101


Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
S Bae, O Mueller, S Wong, JF Rawls, RH Valdivia
Proceedings of the National Academy of Sciences 113 (49), 14127-14132

Molecular Genetic Analysis of Chlamydia Species
BS Sixt, RH Valdivia
Annual Review of Microbiology 70, 179-198

Emancipating Chlamydia: advances in the genetic manipulation of a recalcitrant intracellular pathogen
RJ Bastidas, RH Valdivia
Microbiol. Mol. Biol. Rev. 80 (2), 411-427

Chlamydia Cell biology of the chlamydial inclusion
M Kokes, RH Valdivia
Intracellular Pathogens I: Chlamydiales, 170-191

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We are constantly developing new tools to tackle “genetically intractable” bacteria.  Always open to collaborations to explore the biology of understudied bacteria.



Differential translocation of host cellular materials into the Chlamydia trachomatis inclusion lumen during chemical fixation
M Kokes, RH Valdivia
PloS one 10 (10), e0139153

Global mapping of the Inc-human interactome reveals that retromer restricts Chlamydia infection
KM Mirrashidi, CA Elwell, E Verschueren, JR Johnson, A Frando, …
Cell Host & Microbe 18 (1), 109-121

Integrating chemical mutagenesis and whole-genome sequencing as a platform for forward and reverse genetic analysis of Chlamydia
M Kokes, JD Dunn, JA Granek, BD Nguyen, JR Barker, RH Valdivia, …
Cell Host & Microbe 17 (5), 716-725

Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
HA Saka, JW Thompson, YS Chen, LG Dubois, JT Haas, A Moseley, …
PloS one 10 (4), e0124630


A 2-pyridone-amide inhibitor targets the glucose metabolism pathway of Chlamydia trachomatis
P Engström, KS Krishnan, BD Ngyuen, E Chorell, J Normark, J Silver, …
MBio 6 (1), e02304-14

Reassessing the role of the secreted protease CPAF in Chlamydia trachomatis infection through genetic approaches
EA Snavely, M Kokes, JD Dunn, HA Saka, BD Nguyen, RJ Bastidas, …
Pathogens and Disease 71 (3), 336-351

The Chlamydia trachomatis type III secretion chaperone Slc1 engages multiple early effectors, including TepP, a tyrosine-phosphorylated protein required...
YS Chen, RJ Bastidas, HA Saka, VK Carpenter, KL Richards, GV Plano, …
PLoS pathogens 10 (2), e1003954


The Chlamydia trachomatis inclusion membrane protein CpoS counteracts STING-mediated cellular surveillance and suicide programs
BS Sixt, RJ Bastidas, R Finethy, RM Baxter, VK Carpenter, G Kroemer, …
Cell Host & Microbe 21 (1), 113-121

Mutations in hemG mediate resistance to salicylidene acylhydrazides, demonstrating a novel link between protoporphyrinogen oxidase (HemG) and Chlamydia trachomatis infectivity
P Engström, BD Nguyen, J Normark, I Nilsson, RJ Bastidas, Å Gylfe, …
Journal of Bacteriology 195 (18), 4221-4230

STING-dependent recognition of cyclic di-AMP mediates type I interferon responses during Chlamydia trachomatis infection
JR Barker, BJ Koestler, VK Carpenter, DL Burdette, CM Waters, RE Vance, …
MBio 4 (3), e00018-13

Chlamydial intracellular survival strategies
RJ Bastidas, CA Elwell, JN Engel, RH Valdivia
Cold Spring Harbor Perspectives in Medicine 3 (5), a010256


Emerging roles for lipid droplets in immunity and host-pathogen interactions
HA Saka, R Valdivia
Annual Review of Cell and Developmental Biology 28, 411-437

Virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis revealed by forward genetic approaches
BD Nguyen, RH Valdivia
Proceedings of the National Academy of Sciences 109 (4), 1263-1268

A New Dawn for Chlamydia Research-These important and challenging pathogens are finally yielding to modern analytic techniques
D Rockey, R Valdivia
Microbe 7 (9), 393


Quantitative proteomics reveals metabolic and pathogenic properties of Chlamydia trachomatis developmental forms
HA Saka, JW Thompson, YS Chen, Y Kumar, LG Dubois, MA Moseley, …
Molecular Microbiology 82 (5), 1185-1203

The Chlamydia protease CPAF regulates host and bacterial proteins to maintain pathogen vacuole integrity and promote virulence
I Jorgensen, MM Bednar, V Amin, BK Davis, JPY Ting, DG McCafferty, …
Cell Host & Microbe 10 (1), 21-32

Lipooligosaccharide is required for the generation of infectious elementary bodies in Chlamydia trachomatis
BD Nguyen, D Cunningham, X Liang, X Chen, EJ Toone, CRH Raetz, …
Proceedings of the National Academy of Sciences 108 (25), 10284-10289



cPLA2 regulates the expression of type I interferons and intracellular immunity to Chlamydia trachomatis
MJ Vignola, DF Kashatus, GA Taylor, CM Counter, RH Valdivia
Journal of Biological Chemistry 285 (28), 21625-21635

Acquisition of nutrients by Chlamydiae: unique challenges of living in an intracellular compartment
HA Saka, RH Valdivia
Current Opinion in Microbiology 13 (1), 4-10


New insights into Chlamydia intracellular survival mechanisms
JL Cocchiaro, RH Valdivia
Cellular Microbiology 11 (11), 1571-1578

The Chlamydia type III secretion system C-ring engages a chaperone-effector protein complex
KE Spaeth, YS Chen, RH Valdivia
PLoS pathogens 5 (9), e1000579

Leading a sheltered life: intracellular pathogens and maintenance of vacuolar compartments
Y Kumar, RH Valdivia
Cell Host & Microbe 5 (6), 593-601


Reorganization of the host cytoskeleton by the intracellular pathogen Chlamydia trachomatis
Y Kumar, RH Valdivia
Communicative & Integrative Biology 1 (2), 175-177

Pmp-like proteins Pls1 and Pls2 are secreted into the lumen of the Chlamydia trachomatis inclusion
I Jorgensen, RH Valdivia
Infection and Immunity 76 (9), 3940-3950

Actin and intermediate filaments stabilize the Chlamydia trachomatis vacuole by forming dynamic structural scaffolds
Y Kumar, RH Valdivia
Cell Host & Microbe 4 (2), 159-169

Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole
JL Cocchiaro, Y Kumar, ER Fischer, T Hackstadt, RH Valdivia
Proceedings of the National Academy of Sciences 105 (27), 9379-9384

Chlamydia effector proteins and new insights into chlamydial cellular microbiology
RH Valdivia
Current Opinion in Microbiology 11 (1), 53-59


The obligate intracellular pathogen Chlamydia trachomatis targets host lipid droplets
Y Kumar, J Cocchiaro, RH Valdivia
Current Biology 16 (16), 1646-1651

Multifunctional analysis of Chlamydia‐specific genes in a yeast expression system
JL Sisko, K Spaeth, Y Kumar, RH Valdivia
Molecular Microbiology 60 (1), 51-66

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